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DBA and PNA bound moderately, Con A and UEA-I bound weakly, and LCA bound only slightly to the surface of the corneal epithelium. RCA-I, PHA-E, SNA, WGA, MAA, and SBA reacted Intensely. Lectin (20 microgram/ml) staining was performed on 2 micromiter thickness sections for 8 hrs. The corneas were fixated with 1% glutaraldehyde-1% paraformaldehyde for 24 hrs and then embedded in paraffin. In order to contribute to an understanding of the poorly characterized mucin of the corneal epithelial surface, we investigated the distribution of specific sugar moieties, an important component of mucin, in rabbits using biotinylated-lectin complexes and inhibitory sugars with 11 different lectins(RCA-I, PHA-E, SNA, WGA, MAA, SBA, DBA, PNA, UEA-I, LCA, Con A). It is unique in being composed of a central protein back bone surrounded by an outermost dense oligosaccharide matrix, yet its precise composition is still unknown. The mucous layer located on the cornea epithelial surface is mainly com posed of mucin. 4Department of Biochemistry, Ewha Woman s University College of Medicine, Seoul, Korea. 3Department of Ophtalmology, Inje University Sanggye Paik Hospital, Seoul, Korea. 2Department of Biology, Hallym University, Chuncheon, Korea. 박중원(Jung Won Park),김응권(Eung Kweon Kim),김수진(Su Jin Kim),강신정(Shin Jeong Kang),김형래(Hyung Lae Kim),임지현(Ji Hyun Lim)ġDepartment of Ophtalmology, The Institute of Vision Research, Yonsei University College of Medicine, Seoul, Korea. Lectin Histochemistry of the Mucin on Corneal Epithelial Surface in Rabbit.

Journal of the Korean Ophthalmological Society 1999 40(7):1763-1769.

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